Immobilization and properties of dextransucrase from Leuconostoc mesenteroides culture, LM1.
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چکیده
منابع مشابه
Acceptor reactions and mechanism of Leuconostoc mesenteroides B-512FM dextransucrase
14 INTRODUCTION 16 MATERIALS AND METHODS 19 Carbohydrates and reagents 19 Enzyme 19 Enzyme digest conditions 20 Digest conditions to obtain Hanes-Woolf plots 20 Digest conditions to obtain Michaelis plots 20 Determination of the amount of dextran synthesized 21 RESULTS AND DISCUSSION 22 REFERENCES 3 0 iii PAPER II INTERPRETATION OF DEXTRANSUCRASE INHIBITION AT HIGH SUCROSE CONCENTRATIONS 3 9 AB...
متن کاملPurification and Characterization of Dextransucrase from Leuconostoc Mesenteroides Nrrl B-1149
Leuconostoc mesenteroides NRRL B-1149 produces extracellular dextransucrase which in this study was purified using different concentrations of polyethylene glycol (PEG). The dextran produced by this enzyme is unique in that it contains α(16) and α-(13) linkages which have clinical applications. The cell free supernatant with 0.9 U/mg enzyme specific activity was subjected to fractionation by ...
متن کاملInduction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.
Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nuclear magnetic resonance and high-performance liquid chromatography techniques, r...
متن کاملStudies on Dextransucrase. I. Formation of Riboflavinylglucoside in Destran-producing Cultures of Leuconostoc Mesenteroides.
The dextran produced by this enzymic synthesis has been ascertained by many investigators to be composed largely of ƒ¿-1,6-glucopyranosidic linkage and some of ƒ¿-1 ,4 or ƒ¿-1,3-linkages. Dextransucrase, an enzyme which converts sucrose into dextran as well as fructose approximately equivalent to sucrose as was shown in equation (a), has been found in the cell-free culture fluids of various str...
متن کاملLeuconostoc dextransucrase and dextran: production, properties and applications
This review covers the production, properties and applications of the biopolysaccharide dextran; this biopolymer can be produced via fermentation either with Leuconostoc mesenteroides strains and other lactic acid bacteria or with certain Gluconobacter oxydans strains. The former strains convert sucrose into dextran with the dextransucrase enzyme whereas the latter convert maltodextrins into de...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1995
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.41.399